Tras la celebración de las carreras Bluegreen Vacations Duel, el siguiente sería el orden de salida oficial para la Daytona 500 2020, con la que se inaugura la temporada Cup Series 2020 (domingo, 2:30 PM, Este por FOX, MRN, SiriusXM NASCAR Radio).
Ricky Stenhouse Jr. arrancará desde la Pole Position, seguido por Alex Bowman, quien saldrá desde la primera fila por segunda temporada consecutiva
* equipos open que entraron a la carrera
| Posición | Piloto | Equipo |
| 1. | Ricky Stenhouse Jr. | JTG Daugherty Racing |
| 2. | Alex Bowman | Hendrick Motorsports |
| 3. | Joey Logano | Team Penske |
| 4. | William Byron | Hendrick Motorsports |
| 5. | Aric Almirola | Stewart-Haas Racing |
| 6. | Jimmie Johnson | Hendrick Motorsports |
| 7. | Ryan Newman | Roush Fenway Racing |
| 8. | Kyle Larson | Chip Ganassi Racing |
| 9. | Brad Keselowski | Team Penske |
| 10. | Kevin Harvick | Stewart-Haas Racing |
| 11. | Bubba Wallace | Richard Petty Motorsports |
| 12. | Cole Custer | Stewart-Haas Racing |
| 13 | Austin Dillon | Richard Childress Racing |
| 14. | Erik Jones | Joe Gibbs Racing |
| 15. | Martin Truex Jr. | Joe Gibbs Racing |
| 16. | Matt DiBenedetto | Wood Brothers Racing |
| 17. | Christopher Bell | Leavine Family Racing |
| 18. | Kurt Busch | Chip Ganassi Racing |
| 19. | Chris Buescher | Roush Fenway Racing |
| 20. | Ross Chastain | Spire Motorsports |
| 21. | Denny Hamlin | Joe Gibbs Racing |
| 22. | Tyler Reddick | Richard Childress Racing |
| 23. | John Hunter Nemechek | Front Row Motorsports |
| 24. | Ty Dillon | Germain Racing |
| 25. | Chase Elliott | Hendrick Motorsports |
| 26. | Michael McDowell | Front Row Motorsports |
| 27. | Ryan Blaney | Team Penske |
| 28. | Kyle Busch | Joe Gibbs Racing |
| 29. | Clint Bowyer | Stewart-Haas Racing |
| 30. | David Ragan | Rick Ware Racing |
| 31. | Ryan Preece | JTG Daugherty Racing |
| 32. | Timmy Hill* | MBM Motorsports* |
| 33. | Justin Haley* | Kaulig Racing* |
| 34. | Brennan Poole | Premium Motorsports |
| 35. | Quin Houff | StarCom Racing |
| 36. | Corey LaJoie | GO FAS Racing |
| 37. | Joey Gase | Petty Ware Racing |
| 38. | BJ McLeod | Rick Ware Racing |
| 39. | Brendan Gaughan* | Beard Motorsports* |
| 40. | Reed Sorenson* | Premium Motorsports* |